Cytokinin activity induced by thidiazuron.
نویسندگان
چکیده
The diphenylurea derivative thidiazuron induces a variety of cytokinin responses. Levels above 5 x 10(-9) molar and 4 x 10(-7) molar stimulate maximum soybean callus growth and radish cotyledon expansion, respectively. A wider range of dose response related effects follows thidiazuron induced tobacco plant regeneration. Analysis of soybean callus extracts strongly suggests that thidiazuron treatment creates an accumulation and/or synthesis of purine cytokinins, able to induce the growth, expansion and regeneration, mentioned above.
منابع مشابه
Regulation of Cytokinin Oxidase Activity in Callus Tissues of Phaseolus vulgaris L. cv Great Northern.
The regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern has been examined using an assay based on the oxidation of N(6)-(Delta(2)-isopentenyl)adenine-8-(14)C (i(6) Ade-8-(14)C) to adenine. Solutions of exogenous cytokinins applied directly to the surface of the callus tissues induced relatively rapid increases in cytokinin oxidase activity. The ...
متن کاملEFFECT OF CYTOKININ-ACTIVE PHENYLUREA DERIVATIVES ON SHOOT MULTIPLICATION, PEROXIDASE AND SUPEROXIDE DISMUTASE ACTIVITIES OF in vitro CULTURED CARNATION
In the present study examined the effects of different types of cytokinins on the growth of in vitro cultured carnation. NAA alone produced neither multiplication nor elongation. Combined with BAP, NAA increased multiplication. The biological activity of 4-PU-30 and especially that of thidiazuron was considerably higher than BAP’s activity. In equal concentrations with BAP thidiazuron increased...
متن کاملComparative Transcriptomic Analysis of Vernalization- and Cytokinin-Induced Floral Transition in Dendrobium nobile
Vernalization is required for floral initiation in Dendrobium. Interestingly, those beneficial effects can also be achieved by exogenous cytokinin application in greenhouses. Thus, an as yet unknown crosstalk/interaction may exist between vernalization and cytokinin signaling pathways. In this study, we showed, by de novo transcriptome assembly using RNA-seq data from both vegetative and reprod...
متن کاملMorphoregulatory role of thidiazuron : substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures.
Somatic embryogenesis was induced in hypocotyl explants of geranium (Pelargonium x hortorum) cultured on media supplemented with various concentrations of N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron). In less than 2 weeks, somatic embryos were observed in treatments containing levels of thidiazuron (TDZ) ranging from 0.2 to 1.0 micromolar. The use of N(6)-benzylaminopurine in combination...
متن کاملTopolins and hydroxylated thidiazuron derivatives are substrates of cytokinin O-glucosyltransferase with position specificity related to receptor recognition.
Glucosides of trans-zeatin occur widely in plant tissues, formed either by O-glucosylation of the hydroxylated side chain or N-glucosylation of the purine ring structure. O-Glucosylation is stereo-specific: the O-glucosyltransferase encoded by the Phaseolus lunatus ZOG1 gene has high affinity for trans-zeatin as the substrate, whereas the enzyme encoded by the maize (Zea mays) cisZOG1 gene pref...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Plant physiology
دوره 81 2 شماره
صفحات -
تاریخ انتشار 1986